MARCKS antibody (C-Term)

Catalog No. ABIN2788405

The Rabbit Polyclonal anti-MARCKS antibody is suitable to detect MARCKS in samples from Human, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Pig and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for MARCKS antibody (C-Term) (ABIN2788405)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MARCKS Antibody

Binding Specificity: C-Term

Purpose: MARCKS Antibody - C-terminal region

Sequence: KAAEEPSKVE EKKAEEAGAS AAACEAPSAA GPGAPPEQEA APAEEPAAAA

Predicted Reactivity: Cow: 86%, Human: 100%, Pig: 79%, Rabbit: 86%, Rat: 77%, Yeast: 80%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MARCKS. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MARCKS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60325-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background:

Background Information: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Gene Name: Myristoylated alanine-rich protein kinase C substrate

Alternative Symbols: MACS, 80K-L, PKCSL, PRKCSL

Protein Name: Myristoylated alanine-rich C-kinase substrate

Molecular Weight: 31kDa

Gene ID: 4082

NCBI Accession: NP_002347

UniProt: P29966