MRPL45 antibody (C-Term)

Catalog No. ABIN2788460

This Rabbit Polyclonal antibody specifically detects MRPL45 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for MRPL45 antibody (C-Term) (ABIN2788460)

Target: MRPL45 (Mitochondrial Ribosomal Protein L45 (MRPL45))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL45 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL45 Antibody

Binding Specificity: C-Term

Purpose: MRPL45 Antibody - C-terminal region

Sequence: YGSWRMHTKI VPPWAPPKQP ILKTVMIPGP QLKPEEEYEE AQGEAQKPQL

Predicted Reactivity: Cow: 75%, Human: 100%, Mouse: 91%, Rat: 82%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against MRPL45. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MRPL45

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60476-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MRPL45 antibody (ABIN2788460)

Kotani, Akabane, Takeyasu, Ueda, Takeuchi: "Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes." in: Nucleic acids research, Vol. 41, Issue 6, pp. 3713-22, (2013) (PubMed).

Target Details for MRPL45

Target: MRPL45 (Mitochondrial Ribosomal Protein L45 (MRPL45))

Alternative Name: MRPL45

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.

Gene Name: Mitochondrial ribosomal protein L45

Alternative Symbols: L45mt, MRP-L45

Protein Name: 39S ribosomal protein L45, mitochondrial

Molecular Weight: 35kDa

Gene ID: 84311

NCBI Accession: NP_115727

UniProt: Q9BRJ2