MRPL45 antibody (C-Term)

Catalog No. ABIN2788461

The Rabbit Polyclonal anti-MRPL45 antibody is suitable to detect MRPL45 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse and Rabbit. It has been validated for WB.

Quick Overview for MRPL45 antibody (C-Term) (ABIN2788461)

Target: MRPL45 (Mitochondrial Ribosomal Protein L45 (MRPL45))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL45 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL45 Antibody

Binding Specificity: C-Term

Purpose: MRPL45 Antibody - C-terminal region

Sequence: RFGRLMYGQE DVPKDVLEYV VFEKQLTNPY GSWRMHTKIV PPWAPPKQPI

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MRPL45. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MRPL45

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60477-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL45

Target: MRPL45 (Mitochondrial Ribosomal Protein L45 (MRPL45))

Alternative Name: MRPL45

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.

Gene Name: Mitochondrial ribosomal protein L45

Alternative Symbols: L45mt, MRP-L45

Protein Name: 39S ribosomal protein L45, mitochondrial

Molecular Weight: 35kDa

Gene ID: 84311

NCBI Accession: NP_115727

UniProt: Q9BRJ2