AKR1D1 antibody (Middle Region)

Catalog No. ABIN2788589

This Rabbit Polyclonal antibody specifically detects AKR1D1 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Rabbit, Dog, Horse and Pig.

Quick Overview for AKR1D1 antibody (Middle Region) (ABIN2788589)

Target: AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1D1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKR1D1 Antibody

Binding Specificity: Middle Region

Purpose: AKR1D1 Antibody - middle region

Sequence: YVDLYIIEVP MAFKPGDEIY PRDENGKWLY HKSNLCATWE AMEACKDAGL

Predicted Reactivity: Cow: 86%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Pig: 86%, Rabbit: 100%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against AKR1D1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKR1D1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60804-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for AKR1D1

Target: AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))

Alternative Name: AKR1D1

Background:

Background Information: The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.

Gene Name: Aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)

Alternative Symbols: CBAS2, SRD5B1, 3o5bred

Protein Name: 3-oxo-5-beta-steroid 4-dehydrogenase

Molecular Weight: 37kDa

Gene ID: 6718

NCBI Accession: NP_005980

UniProt: P51857

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process