DOHH antibody (N-Term)

Catalog No. ABIN2788610

The Rabbit Polyclonal anti-DOHH antibody (ABIN2788610) specifically detects DOHH in WB. The antibody is reactive with Human, Cow, Horse, Rat, Dog, Guinea Pig, Mouse, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for DOHH antibody (N-Term) (ABIN2788610)

Target: DOHH (Deoxyhypusine Hydroxylase/monooxygenase (DOHH))

Reactivity: Human, Cow, Horse, Rat, Dog, Guinea Pig, Mouse, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOHH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DOHH Antibody

Binding Specificity: N-Term

Purpose: DOHH Antibody - N-terminal region

Sequence: GQMQDARAIP MLVDVLQDTR QEPMVRHEAG EALGAIGDPE VLEILKQYSS

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 77%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 92%, Rat: 93%, Yeast: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against DOHH. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60855-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DOHH

Target: DOHH (Deoxyhypusine Hydroxylase/monooxygenase (DOHH))

Alternative Name: DOHH

Background:

Background Information: This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.

Gene Name: Deoxyhypusine hydroxylase/monooxygenase

Alternative Symbols: HLRC1, hDOHH

Protein Name: Deoxyhypusine hydroxylase

Molecular Weight: 33kDa

Gene ID: 83475

NCBI Accession: NP_112594

UniProt: Q9BU89