HIST1H3A antibody (N-Term)

Catalog No. ABIN2788642

The Rabbit Polyclonal anti-HIST1H3A antibody is suitable to detect HIST1H3A in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Pig and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for HIST1H3A antibody (N-Term) (ABIN2788642)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIST1H3A Antibody

Binding Specificity: N-Term

Purpose: HIST1H3A Antibody - N-terminal region

Sequence: KQTARKSTGG KAPRKQLATK AARKSAPATG GVKKPHRYRP GTVALREIRR

Predicted Reactivity: Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HIST1H3A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HIST1H3A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60982-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: HIST1H3A

Background:

Background Information: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Gene Name: Histone cluster 1, H3a

Alternative Symbols: H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A

Protein Name: Histone H3.1

Molecular Weight: 15kDa

Gene ID: 8350

NCBI Accession: NP_003520

UniProt: P68431