Histone H3.3 antibody (N-Term)

Catalog No. ABIN2788643

Product Details anti-Histone H3.3 Antibody

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Binding Specificity: N-Term

Reactivity: Rat, Zebrafish (Danio rerio), Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ

Predicted Reactivity: Dog: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against H3f3a. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 126 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3f3a (H3F3A Products)

Background: H3f3a is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. H3f3a constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3f3a is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alias Symbols: H3.3A, H3f3b, MGC102150
Protein Interaction Partner: Kmt2d, Chd5, Setd1a, Kmt2c, Setd1b, Ezh2, Chd1, Cbx3, Uhrf1, Nfkb2, Hdac5, Hdac3, Kdm6b, Nfe2, Aurka, Cbx2, Ehmt2, Cbx5, Aurkb, Cbx6, Bptf, Creb3l4, Ing2, Cbx7, Cbx8, Cbx4, Cd4, Fosb, Rps6ka5, Rps6ka3, Igh-8, Cd79a, Tcrb, Eif2s3x, Kdm5c, Nanog, Kcnq1ot1,
Protein Size: 126

Molecular Weight: 15 kDa

Gene ID: 15078

NCBI Accession: NM_008210, NP_032236

UniProt: P84244