Histone H3.3 antibody (N-Term)

Catalog No. ABIN2788643

The Rabbit Polyclonal anti-Histone H3.3 antibody (ABIN2788643) specifically detects Histone H3.3 in WB. The antibody is reactive with Rat, Pig, Zebrafish (Danio rerio) and Dog samples.

Quick Overview for Histone H3.3 antibody (N-Term) (ABIN2788643)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Rat, Pig, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H3.3 Antibody

Binding Specificity: N-Term

Purpose: H3f3a Antibody - N-terminal region

Sequence: KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ

Predicted Reactivity: Dog: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against H3f3a. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60983-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3f3a

Background:

Background Information: H3f3a is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. H3f3a constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3f3a is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene Name: H3 histone, family 3A

Alternative Symbols: H3-3, H3.3, H3-3a, H3-3b, H3.3A, EyeLinc14

Protein Name: Histone H3.3

Molecular Weight: 15kDa

Gene ID: 15078

NCBI Accession: NP_032236

UniProt: P84244