Topoisomerase I antibody (N-Term)

Catalog No. ABIN2788658

The Rabbit Polyclonal anti-Topoisomerase I antibody (ABIN2788658) specifically detects Topoisomerase I in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit and Dog samples.

Quick Overview for Topoisomerase I antibody (N-Term) (ABIN2788658)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase I Antibody

Binding Specificity: N-Term

Purpose: TOP1 Antibody - N-terminal region

Sequence: SNSEHKDSEK KHKEKEKTKH KDGSSEKHKD KHKDRDKEKR KEEKVRASGD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TOP1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61035-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background:

Background Information: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Gene Name: Topoisomerase (DNA) I

Alternative Symbols: TOPI

Protein Name: DNA topoisomerase 1

Molecular Weight: 91kDa

Gene ID: 7150

NCBI Accession: NP_003277

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance