Hexokinase 1 antibody (N-Term)

Catalog No. ABIN2788663

This anti-Hexokinase 1 antibody is a Rabbit Polyclonal antibody detecting Hexokinase 1 in WB. Suitable for Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio), Horse and Guinea Pig.

Quick Overview for Hexokinase 1 antibody (N-Term) (ABIN2788663)

Target: Hexokinase 1 (HK1)

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio), Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: N-Term

Sequence: CQQSKIDEAI LITWTKRFKA SGVEGADVVK LLNKAIKKRG DYDANIVAVV

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 921 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus, the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
Alias Symbols: HK1-ta, HK1-tb, HK1-tc, HKI, HXK1
Protein Interaction Partner: SUMO2, SUMO3, STAU1, UBC, EGFR, SUMO1, NEDD8, MDM2, WDR61, UBA5, PARP6, NSFL1C, ARFGAP1, UBXN1, TWF2, FKBP9, PDCD10, PROSC, PLAA, GTF3C4, TUFM, TUBB2A, SGTA, RARS, PCNA, HEXB, HEXA, CAPN2, ASL, AHCY, PARK2, ATF2, VDAC1, LIG4, APP, AKT1, CDK2, SIRT7, ICT1,
Protein Size: 921

Molecular Weight: 103 kDa

Gene ID: 3098

NCBI Accession: NM_033498, NP_277033

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect