RAG2 antibody (C-Term)

Catalog No. ABIN2788687

The Rabbit Polyclonal anti-RAG2 antibody has been validated for WB. It is suitable to detect RAG2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for RAG2 antibody (C-Term) (ABIN2788687)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAG2 Antibody

Binding Specificity: C-Term

Sequence: HLSAGSNKYY CNEHVEIARA LHTPQRVLPL KKPPMKSLRK KGSGKILTPA

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RAG2. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 527 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2

Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4.
Alias Symbols: RAG-2
Protein Interaction Partner: RAG1, VPRBP, DDB1, UBC, SKP2, IPO5, KPNA1, tax,
Protein Size: 527

Molecular Weight: 59 kDa

Gene ID: 5897

NCBI Accession: NM_000536, NP_000527

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response