KPNB1 antibody (N-Term)

Catalog No. ABIN2788708

This Rabbit Polyclonal antibody specifically detects KPNB1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for KPNB1 antibody (N-Term) (ABIN2788708)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KPNB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KPNB1 Antibody

Binding Specificity: N-Term

Purpose: KPNB1 Antibody - N-terminal region

Sequence: EHMKESTLEA IGYICQDIDP EQLQDKSNEI LTAIIQGMRK EEPSNNVKLA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KPNB1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61178-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Alternative Name: KPNB1

Background:

Targeted Subunit: beta-1

Background Information: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Gene Name: Karyopherin (importin) beta 1

Alternative Symbols: IMB1, IPO1, IPOB, Impnb, NTF97

Protein Name: Importin subunit beta-1

Molecular Weight: 96kDa

Gene ID: 3837

NCBI Accession: NP_002256

UniProt: Q14974

Pathways: Protein targeting to Nucleus