HMGN3 antibody (Middle Region)

Catalog No. ABIN2788959

This Rabbit Polyclonal antibody specifically detects HMGN3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Dog, Guinea Pig, Rabbit, Cow and Pig.

Quick Overview for HMGN3 antibody (Middle Region) (ABIN2788959)

Target: HMGN3 (High Mobility Group Nucleosomal Binding Domain 3 (HMGN3))

Reactivity: Human, Mouse, Rat, Horse, Dog, Guinea Pig, Rabbit, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGN3 Antibody

Binding Specificity: Middle Region

Purpose: Hmgn3 Antibody - middle region

Sequence: SKPRKTSAKK EPGTKISRGA KGKKEEKQEA GEEGTAPSAN GDTKVEEAQR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against Hmgn3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Hmgn3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61954-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HMGN3

Target: HMGN3 (High Mobility Group Nucleosomal Binding Domain 3 (HMGN3))

Alternative Name: Hmgn3

Background:

Background Information: Hmgn3 binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. It affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion, regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors and regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. It may play a role in ocular development and astrocyte function.

Gene Name: high mobility group nucleosomal binding domain 3

Alternative Symbols: TR, HMGN, TRIP7, BB071015, 1110002A15Rik, 6330514M13Rik

Protein Name: High mobility group nucleosome-binding domain-containing protein 3

Molecular Weight: 11kDa

Gene ID: 94353

NCBI Accession: NP_080398

UniProt: Q9DCB1