BRCC3 antibody (C-Term)

Catalog No. ABIN2788976

This Rabbit Polyclonal antibody specifically detects BRCC3 in WB and IF. It exhibits reactivity toward Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig and Rabbit.

Quick Overview for BRCC3 antibody (C-Term) (ABIN2788976)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivity: Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-BRCC3 Antibody

Binding Specificity: C-Term

Sequence: HNGSVFTKNL CSQMSAVSGP LLQWLEDRLE QNQQHLQELQ QEKEELMQEL

Predicted Reactivity: Cow: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against BRCC3. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 316 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Alternative Name: BRCC3

Background: This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy.
Alias Symbols: BRCC36, C6.1A, CXorf53, RP11-143H17.2
Protein Interaction Partner: FAM175B, UBC, FANCG, UIMC1, SUMO1, RABEP2, SMEK1, LARP7, CD2AP, WDHD1, SF3A1, GPHN, HNRNPR, TRIM28, HUWE1, MTA2, SPAG9, CCDC6, HDAC1, NR3C1, FAM175A, SUMO2, PAXIP1, MDC1, BRCA1, GRB2, BABAM1, BRE, BRCC3, COPS5, BACH1, BRCA2, BARD1, USP7, SHMT2, NDUFA10, M
Protein Size: 316

Molecular Weight: 35 kDa

Gene ID: 79184

NCBI Accession: NM_024332, NP_077308

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus