RASGRP4 antibody (N-Term)

Catalog No. ABIN2789029

This Rabbit Polyclonal antibody specifically detects RASGRP4 in WB. It exhibits reactivity toward Human and Pig.

Quick Overview for RASGRP4 antibody (N-Term) (ABIN2789029)

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRP4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRP4 Antibody

Binding Specificity: N-Term

Purpose: RASGRP4 Antibody - N-terminal region

Sequence: FWATVAREGN SAQRRLGDSS DLLSPGGPGP PLPMSSPGLG KKRKVSLLFD

Predicted Reactivity: Human: 100%, Pig: 92%

Characteristics: This is a rabbit polyclonal antibody against RASGRP4. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP62124-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RASGRP4

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Alternative Name: RASGRP4

Background:

Background Information: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: RAS guanyl releasing protein 4

Alternative Symbols: -

Protein Name: RAS guanyl-releasing protein 4

Molecular Weight: 63kDa

Gene ID: 115727

NCBI Accession: NP_001139679

UniProt: C0LTP7

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling