SPARC antibody (C-Term)

Catalog No. ABIN2789396

Product Details anti-SPARC Antibody

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALDEWAGCFG IKQKDIDKDL

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 93%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 92%, Sheep: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against SPARC. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 303 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPARC antibody (ABIN2789396)

Boyineni, Tanpure, Gnanamony, Antony, Fernández, Lin, Pinson, Gondi: "SPARC overexpression combined with radiation retards angiogenesis by suppressing VEGF-A via miR‑410 in human neuroblastoma cells." in: International journal of oncology, Vol. 49, Issue 4, pp. 1394-406, (2016) (PubMed).

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC (SPARC Products)

Synonyms: ON antibody, AA517111 antibody, BM-40 antibody, hm:zeh0062 antibody, SPARC antibody, sparc antibody, secreted protein acidic and cysteine rich antibody, secreted acidic cysteine rich glycoprotein antibody, secreted protein, acidic, cysteine-rich (osteonectin) antibody, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog antibody, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog antibody, SPARC antibody, Sparc antibody, sparc antibody, sparc.L antibody, sparc.S antibody

Background: Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
Alias Symbols: ON
Protein Interaction Partner: UBQLN1, ZNF579, XRCC6, CTSK, VEGFA, TGM2, TGFB1, THBS1, SPARC, PDGFA, PLAT, PLG, HSPG2, SDC2, FN1, COL13A1, COL3A1, COL1A2, COL1A1, COL2A1, PDGFB, COL5A1,
Protein Size: 303

Molecular Weight: 35 kDa

Gene ID: 6678

NCBI Accession: NM_003118, NP_003109

UniProt: P09486

Pathways: Autophagy