SPARC antibody (C-Term)

Catalog No. ABIN2789396

This Rabbit Polyclonal antibody specifically detects SPARC in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Sheep, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig and Horse.It has been mentioned in 1 publication

Quick Overview for SPARC antibody (C-Term) (ABIN2789396)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Sheep, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPARC Antibody

Binding Specificity: C-Term

Purpose: SPARC Antibody - C-terminal region

Sequence: LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALDEWAGCFG IKQKDIDKDL

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 93%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 92%, Sheep: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against SPARC. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63168-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPARC antibody (ABIN2789396)

Boyineni, Tanpure, Gnanamony, Antony, Fernández, Lin, Pinson, Gondi: "SPARC overexpression combined with radiation retards angiogenesis by suppressing VEGF-A via miR‑410 in human neuroblastoma cells." in: International journal of oncology, Vol. 49, Issue 4, pp. 1394-406, (2016) (PubMed).

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background:

Background Information: Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

Gene Name: Secreted protein, acidic, cysteine-rich (osteonectin)

Alternative Symbols: ON, ONT, OI17, BM-40

Protein Name: SPARC

Molecular Weight: 35kDa

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy