PRNP antibody (Middle Region)

Catalog No. ABIN2789398

This Rabbit Polyclonal antibody specifically detects PRNP in WB and IHC. It exhibits reactivity toward Human, Rat, Cow, Sheep, Dog and Goat.

Quick Overview for PRNP antibody (Middle Region) (ABIN2789398)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human, Rat, Cow, Sheep, Dog, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRNP Antibody

Binding Specificity: Middle Region

Purpose: Prnp Antibody - middle region

Sequence: GGGTHNQWNK PSKPKTNLKH VAGAAAAGAV VGGLGGYMLG SAMSRPMIHF

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Human: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against Prnp. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Prnp

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63174-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: Prnp

Background:

Background Information: Prnp may play a role in neuronal development and synaptic plasticity, may be required for neuronal myelin sheath maintenance and may play a role in iron uptake and iron homeostasis. Prnp is a soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. It also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.

Gene Name: prion protein

Alternative Symbols: P, S, PrP, PrPC, Sinc, CD230, PrPSc, Prn-i, Prn-p, PrP, AA960666, AI325101, prP27-30, prP33-35C

Protein Name: Major prion protein

Molecular Weight: 28kDa

Gene ID: 19122

NCBI Accession: NP_035300

UniProt: P04925

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation