MCAM antibody (Middle Region)

Catalog No. ABIN2789518

This Rabbit Polyclonal antibody specifically detects MCAM in WB. It exhibits reactivity toward Human, Rabbit, Pig, Horse and Cow.

Quick Overview for MCAM antibody (Middle Region) (ABIN2789518)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Rabbit, Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCAM Antibody

Binding Specificity: Middle Region

Purpose: MCAM Antibody - middle region

Sequence: PSTREAEEET TNDNGVLVLE PARKEHSGRY ECQGLDLDTM ISLLSEPQEL

Predicted Reactivity: Cow: 86%, Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 79%

Characteristics: This is a rabbit polyclonal antibody against MCAM. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63483-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background:

Background Information: MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. MCAM could be an adhesion molecule active in neural crest cells during embryonic development. MCAM acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Gene Name: Melanoma cell adhesion molecule

Alternative Symbols: CD146, MUC18, HEMCAM, METCAM, MelCAM

Protein Name: Cell surface glycoprotein MUC18

Molecular Weight: 71kDa

Gene ID: 4162

NCBI Accession: NP_006491

UniProt: P43121