HCAR2 antibody (C-Term)

Catalog No. ABIN2789654

Product Details anti-HCAR2 Antibody

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCAR2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YYFSSPSFPN FFSTLINRCL QRKMTGEPDN NRSTSVELTG DPNKTRGAPE

Predicted Reactivity: Cow: 91%, Guinea Pig: 92%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HCAR2. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 363 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HCAR2

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Alternative Name: HCAR2 (HCAR2 Products)

Synonyms: GPR109A antibody, HCA2 antibody, HM74a antibody, HM74b antibody, NIACR1 antibody, PUMAG antibody, Puma-g antibody, Gpr109a antibody, Gpr109b antibody, HM74 antibody, Hcar2 antibody, PUMA-G antibody, Pumag antibody, mHM74b antibody, HCAR2 antibody, HCAR3 antibody, hydroxycarboxylic acid receptor 2 antibody, HCAR2 antibody, Hcar2 antibody, LOC527744 antibody

Background: HCAR2 acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
Alias Symbols: HM74a, HM74b, NIACR1, PUMAG, Puma-g
Protein Size: 363

Molecular Weight: 42 kDa

Gene ID: 338442

NCBI Accession: NM_177551, NP_808219

UniProt: Q8TDS4