NCK1 antibody (N-Term)

Catalog No. ABIN2789859

The Rabbit Polyclonal anti-NCK1 antibody is suitable to detect NCK1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for NCK1 antibody (N-Term) (ABIN2789859)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCK1 Antibody

Binding Specificity: N-Term

Purpose: NCK1 Antibody - N-terminal region

Sequence: PSNYVERKNS ARKASIVKNL KDTLGIGKVK RKPSVPDSAS PADDSFVDPG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against NCK1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP64517-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background:

Background Information: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Gene Name: NCK adaptor protein 1

Alternative Symbols: NCK, nck-1, NCKalpha

Protein Name: Cytoplasmic protein NCK1

Molecular Weight: 42kDa

Gene ID: 4690

NCBI Accession: NP_006144

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals