EIF3I antibody (C-Term)

Catalog No. ABIN2789974

The Rabbit Polyclonal anti-EIF3I antibody has been validated for WB, IHC and IP. It is suitable to detect EIF3I in samples from Human, Mouse, Rat, Dog, Horse, Rabbit, Cow and Guinea Pig.

Quick Overview for EIF3I antibody (C-Term) (ABIN2789974)

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-EIF3I Antibody

Binding Specificity: C-Term

Sequence: NVKEHSRQIN DIQLSRDMTM FVTASKDNTA KLFDSTTLEH QKTFRTERPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF3I. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 325 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3I

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Alternative Name: EIF3I

Background: EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Alias Symbols: EIF3S2, PRO2242, TRIP-1, TRIP1, eIF3-beta, eIF3-p36
Protein Interaction Partner: HUWE1, UBC, SUMO2, SUMO3, RNF2, rev, EIF3G, HECW2, EIF3B, CDK18, TARDBP, NPM1, ICAM1, CD81, IGSF8, PAN2, EIF3D, VCAM1, ITGA4, METTL18, gag-pol, DDX3X, PAXIP1, LIG4, EIF3L, EIF3K, EIF3M, EIF3H, EIF3F, EIF3C, EIF3A, EIF3E, CUL3, CDK2, YWHAZ, SIRT7, ARRB1, R
Protein Size: 325

Molecular Weight: 36 kDa

Gene ID: 8668

NCBI Accession: NM_003757, NP_003748

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA