NANS antibody (C-Term)

Catalog No. ABIN2790121

This Rabbit Polyclonal antibody specifically detects NANS in WB. It exhibits reactivity toward Human, Rat, Cow, Dog, Rabbit and Pig.

Quick Overview for NANS antibody (C-Term) (ABIN2790121)

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Reactivity: Human, Rat, Cow, Dog, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NANS Antibody

Binding Specificity: C-Term

Purpose: NANS Antibody - C-terminal region

Sequence: DMLTVKVGEP KGYPPEDIFN LVGKKVLVTV EEDDTIMEEL VDNHGKKIKS

Predicted Reactivity: Cow: 86%, Dog: 79%, Human: 100%, Pig: 86%, Rabbit: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against NANS. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65353-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NANS

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Alternative Name: NANS

Background:

Background Information: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively, however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Gene Name: N-acetylneuraminic acid synthase

Alternative Symbols: SAS, SEMDG, SEMDCG, HEL-S-100

Protein Name: Sialic acid synthase

Molecular Weight: 40kDa

Gene ID: 54187

NCBI Accession: NP_061819

UniProt: Q9NR45