RDH8 antibody (Middle Region)

Catalog No. ABIN2790129

This Rabbit Polyclonal antibody specifically detects RDH8 in WB. It exhibits reactivity toward Human, Mouse, Pig, Rabbit, Rat, Cow, Dog, Guinea Pig and Horse.

Quick Overview for RDH8 antibody (Middle Region) (ABIN2790129)

Target: RDH8 (Retinol Dehydrogenase 8 (RDH8))

Reactivity: Human, Mouse, Pig, Rabbit, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RDH8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RDH8 Antibody

Binding Specificity: Middle Region

Purpose: RDH8 Antibody - middle region

Sequence: FDTNFFGAVR LVKAVLPGMK RRRQGHIVVI SSVMGLQGVI FNDVYAASKF

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RDH8. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human RDH8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65373-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RDH8

Target: RDH8 (Retinol Dehydrogenase 8 (RDH8))

Alternative Name: RDH8

Background:

Background Information: All-trans-retinol dehydrogenase (RDH8) is a visual cycle enzyme that reduces all-trans-retinal to all-trans-retinol in the presence of NADPH (Rattner et al., 2000 [PubMed 10753906]). It is a member of the short chain dehydrogenase/reductase family and is located in the outer segments of photoreceptors, hence it is also known as photoreceptor retinol dehydrogenase. It is important in the visual cycle by beginning the rhodopsin regeneration pathway by reducing all-trans-retinal, the product of bleached and hydrolysed rhodopsin.

Gene Name: Retinol dehydrogenase 8 (all-trans)

Alternative Symbols: PRRDH, SDR28C2

Protein Name: Retinol dehydrogenase 8

Molecular Weight: 34 kDa

Gene ID: 50700

NCBI Accession: NP_056540

UniProt: Q9NYR8