CH25H antibody (C-Term)

Catalog No. ABIN2790174

The Rabbit Polyclonal anti-CH25H antibody is suitable to detect CH25H in samples from Human, Mouse, Cow, Goat, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for CH25H antibody (C-Term) (ABIN2790174)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Human, Mouse, Cow, Goat, Guinea Pig, Horse, Rabbit, Rat, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CH25H Antibody

Binding Specificity: C-Term

Purpose: CH25H Antibody - C-terminal region

Sequence: VTLLGCHPLT TLTFHVVNIW LSVEDHSGYN FPWSTHRLVP FGWYGGVVHH

Predicted Reactivity: Cow: 93%, Goat: 83%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Yeast: 75%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CH25H. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human CH25H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65536-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background:

Background Information: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.

Gene Name: Cholesterol 25-hydroxylase

Alternative Symbols: C25H

Protein Name: Cholesterol 25-hydroxylase

Molecular Weight: 32 kDa

Gene ID: 9023

NCBI Accession: NP_003947

UniProt: O95992