SPAG1 antibody (N-Term)

Catalog No. ABIN2790231

The Rabbit Polyclonal anti-SPAG1 antibody has been validated for WB. It is suitable to detect SPAG1 in samples from Human and Dog.

Quick Overview for SPAG1 antibody (N-Term) (ABIN2790231)

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPAG1 Antibody

Binding Specificity: N-Term

Sequence: LHVGKEKYSK RPTKKKTPRD YAEWDKFDVE KECLKIDEDY KEKTVIDKSH

Predicted Reactivity: Dog: 85%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against SPAG1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of human SPAG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPAG1

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Alternative Name: SPAG1

Background: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.
Alias Symbols: SP75, TPIS, CT140, CILD28, HSD-3.8, HEL-S-268
Protein Size: 416

Molecular Weight: 45 kDa

Gene ID: 6674

NCBI Accession: NM_003114, NP_003105

UniProt: G3XAM3