CHMP5 antibody (C-Term)

Catalog No. ABIN2790285

The Rabbit Polyclonal anti-CHMP5 antibody is suitable to detect CHMP5 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for CHMP5 antibody (C-Term) (ABIN2790285)

Target: CHMP5 (Charged Multivesicular Body Protein 5 (CHMP5))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHMP5 Antibody

Binding Specificity: C-Term

Purpose: Chmp5 Antibody - C-terminal region

Sequence: LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against Chmp5. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Chmp5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65870-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHMP5

Target: CHMP5 (Charged Multivesicular Body Protein 5 (CHMP5))

Alternative Name: Chmp5

Background:

Background Information: Chmp5 is a probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.

Gene Name: charged multivesicular body protein 5

Alternative Symbols: AW545668, 2210412K09Rik

Protein Name: Charged multivesicular body protein 5

Molecular Weight: 24kDa

Gene ID: 76959

NCBI Accession: NP_084090

UniProt: Q9D7S9