DPAGT1 antibody (C-Term)

Catalog No. ABIN2790329

This Rabbit Polyclonal antibody specifically detects DPAGT1 in WB. It exhibits reactivity toward Human, Mouse, Dog, Guinea Pig, Horse, Rabbit and Rat.

Quick Overview for DPAGT1 antibody (C-Term) (ABIN2790329)

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPAGT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DPAGT1 Antibody

Binding Specificity: C-Term

Purpose: DPAGT1 Antibody - C-terminal region

Sequence: TKSLSFLGTF ILKVAESLQL VTVHQSETED GEFTECNNMT LINLLLKVLG

Specificity: Isoform 1 (44 kDa), 2 (44 kDa), 3(33 kDa)

Predicted Reactivity: Dog: 86%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 77%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against DPAGT1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human DPAGT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP66022-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DPAGT1

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Alternative Name: DPAGT1

Background:

Background Information: The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme.

Alternative Symbols: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, CMS13, DPAGT, CDG-Ij, CMSTA2, DPAGT2, D11S366

Protein Name: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Molecular Weight: 33kDa

Gene ID: 1798

NCBI Accession: NP_001373

UniProt: Q9H3H5