MED20 antibody (Middle Region)

Catalog No. ABIN2790334

This Rabbit Polyclonal antibody specifically detects MED20 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse and Rabbit.

Quick Overview for MED20 antibody (Middle Region) (ABIN2790334)

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Reactivity: Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED20 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED20 Antibody

Binding Specificity: Middle Region

Sequence: PCLIADTNFD VLMVKLKGFF QSAKASKIET RGTRYQYCDF LVKVGTVTMG

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MED20. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MED20

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 212 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED20

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Alternative Name: MED20

Background: MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors.
Alias Symbols: DKFZp586D2223, MGC29869, PRO0213, TRFP
Protein Interaction Partner: MED18, UBC, CDK8, CDK19, MED19, POLR1B, CD300C, MED26, EPAS1, FBXW7, ELAVL1, MED10, MED28, ZC3H13, MED12, TADA2A, MED1, MED29, MED8, MED30, ESR2, ESR1, PPARGC1A, MED9,
Protein Size: 212

Molecular Weight: 23 kDa

Gene ID: 9477

NCBI Accession: NM_004275, NP_004266

UniProt: Q9H944

Pathways: Regulation of Lipid Metabolism by PPARalpha