MED20 antibody (AA 60-140)

Catalog No. ABIN7229624

The Rabbit Polyclonal anti-MED20 antibody is suitable to detect MED20 in samples from Human, Rat and Mouse. It has been validated for ELISA and WB.

Quick Overview for MED20 antibody (AA 60-140) (ABIN7229624)

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED20 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MED20 Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-MED20 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MED20 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MED20 protein at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED20

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Alternative Name: MED20

Background: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFPMED20 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.

Molecular Weight: 23kD

Gene ID: 9477

UniProt: Q9H944

Pathways: Regulation of Lipid Metabolism by PPARalpha