LGMN antibody (Middle Region)

Catalog No. ABIN2790480

This Rabbit Polyclonal antibody specifically detects LGMN in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for LGMN antibody (Middle Region) (ABIN2790480)

Target: LGMN (Legumain (LGMN))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGMN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LGMN Antibody

Binding Specificity: Middle Region

Sequence: KMVFYIEACE SGSMMNHLPD NINVYATTAA NPRESSYACY YDEKRSTYLG

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against LGMN. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human LGMN

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 433 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LGMN

Target: LGMN (Legumain (LGMN))

Alternative Name: LGMN

Background: This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
Alias Symbols: AEP, LGMN1, PRSC1
Protein Interaction Partner: USP17L2, TRAF6, USP17L9P, SUMO1, UBC, NEDD8, LAMC3, ACIN1, IGF2BP3, HNRNPR, FBXO6, ISG15, LGMN,
Protein Size: 433

Molecular Weight: 48 kDa

Gene ID: 5641

NCBI Accession: NM_005606, NP_005597

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades