CCDC99 antibody (C-Term)

Catalog No. ABIN2790740

The Rabbit Polyclonal anti-CCDC99 antibody (ABIN2790740) specifically detects CCDC99 in WB. The antibody is reactive with Human, Rat, Cow, Dog, Horse, Pig and Rabbit samples.

Quick Overview for CCDC99 antibody (C-Term) (ABIN2790740)

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Reactivity: Human, Rat, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCDC99 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCDC99 Antibody

Binding Specificity: C-Term

Purpose: CCDC99 Antibody - C-terminal region

Sequence: PRLAAESKLQ TEVKEGKETS SKLEKETCKK LHPILYVSSK STPETQCPQQ

Predicted Reactivity: Cow: 79%, Dog: 93%, Horse: 92%, Human: 100%, Pig: 93%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against CCDC99. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human CCDC99

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP67438-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CCDC99

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Alternative Name: CCDC99

Background:

Background Information: CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. CCDC99 is also required for correct spindle orientation. It does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.

Alternative Symbols: CCDC99

Protein Name: Protein Spindly

Molecular Weight: 67kDa

Gene ID: 54908

NCBI Accession: NP_060255

UniProt: Q96EA4