YOD1 antibody (N-Term)

Catalog No. ABIN2790891

The Rabbit Polyclonal anti-YOD1 antibody is suitable to detect YOD1 in samples from Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Cow. It has been validated for WB.

Quick Overview for YOD1 antibody (N-Term) (ABIN2790891)

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YOD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YOD1 Antibody

Binding Specificity: N-Term

Purpose: Yod1 Antibody - N-terminal region

Sequence: VPQSEAGTKA GPAGGRPADT MWRVRCKAKG GTHLLQGLSS RTRLRELQGQ

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against Yod1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of MOUSE Yod1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP67915-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for YOD1

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Alternative Name: Yod1

Background:

Background Information: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. Yod1 may act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Yod1 mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. It is able to cleave both polyubiquitin and di-ubiquitin.

Gene Name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)

Alternative Symbols: Hshin7, 6330564D18Rik, 9930028C20Rik

Protein Name: Ubiquitin thioesterase OTU1

Molecular Weight: 38 kDa

Gene ID: 226418

NCBI Accession: NP_848806

UniProt: Q8CB27

Pathways: ER-Nucleus Signaling