ATP6V0E2 antibody (N-Term)

Catalog No. ABIN2790913

The Rabbit Polyclonal anti-ATP6V0E2 antibody is suitable to detect ATP6V0E2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for ATP6V0E2 antibody (N-Term) (ABIN2790913)

Target: ATP6V0E2 (ATPase, H+ Transporting V0 Subunit E2 (ATP6V0E2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0E2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V0E2 Antibody

Binding Specificity: N-Term

Purpose: ATP6V0E2 Antibody - N-terminal region

Sequence: GPWFVPKGPN RGVIITMLVA TAVCCYLFWL IAILAQLNPL FGPQLKNETI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0E2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ATP6V0E2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP67982-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6V0E2

Target: ATP6V0E2 (ATPase, H+ Transporting V0 Subunit E2 (ATP6V0E2))

Alternative Name: ATP6V0E2

Background:

Background Information: Multisubunit vacuolar-type proton pumps, or H(+)-ATPases, acidify various intracellular compartments, such as vacuoles, clathrin-coated and synaptic vesicles, endosomes, lysosomes, and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells, where they play roles in urinary acidification, bone resorption, and sperm maturation. Multiple subunits form H(+)-ATPases, with proteins of the V1 class hydrolyzing ATP for energy to transport H+, and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit, an essential proton pump component.

Gene Name: ATPase, H+ transporting, lysosomal 9 kDa, V0 subunit e1

Alternative Symbols: C7orf32, ATP6V0E2L

Protein Name: V-type proton ATPase subunit e 2

Molecular Weight: 20kDa

Gene ID: 155066

NCBI Accession: NP_660265

UniProt: Q8NHE4

Pathways: Transition Metal Ion Homeostasis, Proton Transport