ANAPC15 antibody (N-Term)

Catalog No. ABIN2790945

This Rabbit Polyclonal antibody specifically detects ANAPC15 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for ANAPC15 antibody (N-Term) (ABIN2790945)

Target: ANAPC15 (Anaphase Promoting Complex Subunit 15 (ANAPC15))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANAPC15 Antibody

Binding Specificity: N-Term

Purpose: ANAPC15 Antibody - N-terminal region

Sequence: EETELQQQEQ QHQAWLQSIA EKDNNLVPIG KPASEHYDDE EEEDDEDDED

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 90%

Characteristics: This is a rabbit polyclonal antibody against ANAPC15. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ANAPC15

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP68090-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ANAPC15

Target: ANAPC15 (Anaphase Promoting Complex Subunit 15 (ANAPC15))

Alternative Name: ANAPC15

Background:

Targeted Subunit: 15

Background Information: ANAPC15 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. In the complex, it plays a role in the release of the mitotic checkpoint complex (MCC) from the APC/C: not required for APC/C activity itself, but promotes the turnover of CDC20 and MCC on the APC/C, thereby participating to the responsiveness of the spindle assembly checkpoint. It is also required for degradation of CDC20.

Alternative Symbols: APC15, HSPC020, C11orf51

Protein Name: Anaphase-promoting complex subunit 15

Molecular Weight: 14 kDa

Gene ID: 25906

NCBI Accession: NP_054761

UniProt: P60006