Anaphase Promoting Complex Subunit 15 (ANAPC15) (N-Term) antibody

Details for Product No. ABIN2790945
Antigen
Epitope
N-Term
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
12
10
10
1
1
1
1
1
1
Host
Rabbit
12
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Western Blotting (WB)
7
4
4
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the N-terminal region of Human ANAPC15
Blocking Peptide Blocking peptide for this product available: ABIN1541955
Sequence EETELQQQEQ QHQAWLQSIA EKDNNLVPIG KPASEHYDDE EEEDDEDDED
Predicted Reactivity Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 90%
Characteristics This is a rabbit polyclonal antibody against ANAPC15. It was validated on Western Blot.
Purification Affinity Purified
Antigen
Alternative Name ANAPC15 (ANAPC15 Antibody Abstract)
Background ANAPC15 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. In the complex, it plays a role in the release of the mitotic checkpoint complex (MCC) from the APC/C: not required for APC/C activity itself, but promotes the turnover of CDC20 and MCC on the APC/C, thereby participating to the responsiveness of the spindle assembly checkpoint. It is also required for degradation of CDC20.
Alias Symbols: APC15, C11orf51
Protein Interaction Partner: ANAPC4, CDC26, ANAPC1, ANAPC7, ANAPC5, ANAPC2, ANAPC10, CDC16, CDC23, CDC27, CDC20,
Protein Size: 121
Molecular Weight 13 kDa
Gene ID 25906
NCBI Accession NM_014042, NP_054761
UniProt P60006
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 121 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Anaphase Promoting Complex Subunit 15 (ANAPC15) (N-Term) antibody (ABIN2790945) Host: Rabbit Target Name: ANAPC15 Sample Type: HT1080 Whole Cell lysates Antibody Dil...