C12orf65 antibody (C-Term)

Catalog No. ABIN2790950

The Rabbit Polyclonal anti-C12orf65 antibody is suitable to detect C12orf65 in samples from Human, Mouse, Cow, Dog, Guinea Pig, Horse, Pig and Rat. It has been validated for WB.

Quick Overview for C12orf65 antibody (C-Term) (ABIN2790950)

Target: C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C12orf65 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C12orf65 Antibody

Binding Specificity: C-Term

Purpose: C12orf65 Antibody - C-terminal region

Sequence: HIPSGIVVKC HQTRSVDQNR KLARKILQEK VDVFYNGENS PVHKEKREAA

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against C12orf65. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human C12orf65

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP68112-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for C12orf65

Target: C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))

Alternative Name: C12orf65

Background:

Background Information: This nuclear gene encodes a mitochondrial matrix protein that appears to contribute to peptide chain termination in the mitochondrial translation machinery. Two different 1 bp deletions (resulting in the same premature stop codon)result in decreased mitochondrial translation, decreased levels of oxidative phosphorylation complexes and encepthalomyopathy. Alternative splicing results in multiple transcript variants.

Alternative Symbols: SPG55, COXPD7, C12orf65

Protein Name: Probable peptide chain release factor C12orf65, mitochondrial

Molecular Weight: 18kDa

Gene ID: 91574

NCBI Accession: NP_689482

UniProt: Q9H3J6