POLR3G antibody (Middle Region)
-
- Target See all POLR3G Antibodies
- POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))
-
Binding Specificity
- Middle Region
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This POLR3G antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- SKRYMKVYKE EWIPDWRRLP REMMPRNKCK KAGPKPKKAK DAGKGTPLTN
- Predicted Reactivity
- Human: 100%
- Characteristics
- This is a rabbit polyclonal antibody against POLR3G. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of Human POLR3G
- Top Product
- Discover our top product POLR3G Primary Antibody
-
-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Antigen size: 223 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))
- Alternative Name
- POLR3G (POLR3G Products)
- Synonyms
- 2310047G20Rik antibody, AV275904 antibody, RPC32 antibody, RPC7 antibody, polymerase (RNA) III (DNA directed) polypeptide G antibody, RNA polymerase III subunit G antibody, Polr3g antibody, POLR3G antibody
- Background
-
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3G is a specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It may direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. It may be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. POLR3G plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3G acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3G can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Alias Symbols: RPC32, RPC7
Protein Interaction Partner: RBM28, POLR3C, POLR3F, POLR2C, UBC, POLR3H, POLR3D,
Protein Size: 223 - Molecular Weight
- 26 kDa
- Gene ID
- 10622
- NCBI Accession
- NM_006467, NP_006458
- UniProt
- O15318
-