MRPL14 antibody (C-Term)

Catalog No. ABIN2791154

The Rabbit Polyclonal anti-MRPL14 antibody (ABIN2791154) specifically detects MRPL14 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig and Zebrafish (Danio rerio) samples.

Quick Overview for MRPL14 antibody (C-Term) (ABIN2791154)

Target: MRPL14 (Mitochondrial Ribosomal Protein L14 (MRPL14))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL14 Antibody

Binding Specificity: C-Term

Purpose: MRPL14 Antibody - C-terminal region

Sequence: MTPRFDSNNV VLIEDNGNPV GTRIKTPIPT SLRKREGEYS KVLAIAQNFV

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 86%, Horse: 86%, Human: 100%, Mouse: 93%, Pig: 79%, Rat: 93%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against MRPL14. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MRPL14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP68816-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL14

Target: MRPL14 (Mitochondrial Ribosomal Protein L14 (MRPL14))

Alternative Name: MRPL14

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3.

Alternative Symbols: L14mt, L32mt, MRPL32, RMPL32, RPML32, MRP-L14, MRP-L32

Protein Name: 39S ribosomal protein L14, mitochondrial

Molecular Weight: 15kDa

Gene ID: 64928

NCBI Accession: NP_115487

UniProt: Q6P1L8