MRPL18 antibody (N-Term)

Catalog No. ABIN2791669

This Rabbit Polyclonal antibody specifically detects MRPL18 in WB. It exhibits reactivity toward Human, Dog, Rabbit and Rat.

Quick Overview for MRPL18 antibody (N-Term) (ABIN2791669)

Target: MRPL18 (Mitochondrial Ribosomal Protein L18 (MRPL18))

Reactivity: Human, Dog, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL18 Antibody

Binding Specificity: N-Term

Purpose: MRPL18 Antibody - N-terminal region

Sequence: RNPGCRFAAL STSSEPAAKP EVDPVENEAV APEFTNRNPR NLELLSVARK

Predicted Reactivity: Dog: 93%, Human: 100%, Rabbit: 79%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against MRPL18. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human MRPL18

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP70881-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL18

Target: MRPL18 (Mitochondrial Ribosomal Protein L18 (MRPL18))

Alternative Name: MRPL18

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus.

Alternative Symbols: L18mt, HSPC071, MRP-L18

Protein Name: 39S ribosomal protein L18, mitochondrial

Molecular Weight: 20kDa

Gene ID: 29074

NCBI Accession: NP_054880

UniProt: Q9H0U6