MRPL30 antibody (C-Term)

Catalog No. ABIN2791670

The Rabbit Polyclonal anti-MRPL30 antibody (ABIN2791670) specifically detects MRPL30 in WB. The antibody is reactive with Human, Mouse, Rat, Dog and Rabbit samples.

Quick Overview for MRPL30 antibody (C-Term) (ABIN2791670)

Target: MRPL30 (Mitochondrial Ribosomal Protein L30 (MRPL30))

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL30 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL30 Antibody

Binding Specificity: C-Term

Purpose: MRPL30 Antibody - C-terminal region

Sequence: VVKHLIRIKP LKLPQGLPAE ENMSNTCLKS TGELVVQWHL KPVEQKAHES

Predicted Reactivity: Dog: 80%, Human: 100%, Mouse: 91%, Rabbit: 89%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against MRPL30. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MRPL30

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP70883-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL30

Target: MRPL30 (Mitochondrial Ribosomal Protein L30 (MRPL30))

Alternative Name: MRPL30

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.

Alternative Symbols: L28MT, L30MT, MRPL28, RPML28, MRP-L28, MRP-L30, MRPL28M

Protein Name: 39S ribosomal protein L30, mitochondrial

Molecular Weight: 14kDa

Gene ID: 51263

NCBI Accession: NP_660213

UniProt: Q8TCC3