SPTA1 antibody (N-Term)

Catalog No. ABIN2792075

The Rabbit Polyclonal anti-SPTA1 antibody (ABIN2792075) specifically detects SPTA1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse and Rabbit samples.

Quick Overview for SPTA1 antibody (N-Term) (ABIN2792075)

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPTA1 Antibody

Binding Specificity: N-Term

Sequence: DELSGWMNEK TAAINADELP TDVAGGEVLL DRHQQHKHEI DSYDDRFQSA

Predicted Reactivity: Cow: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPTA1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SPTA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPTA1

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Alternative Name: SPTA1

Background: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia.
Alias Symbols: EL2, HPP, HS3, SPH3, SPTA
Protein Interaction Partner: ANK1, PTPRO, UBC, YWHAQ, MYBL2, PTPN22, BTK, UBASH3B, SHC1, CBL, FASLG, NCOA3, ENAH, EVL, SPTBN2, ABI1, VASP, PLEC, SLC9A2, FANCC, SPTB, ADD1, ACTA1, ABL1, EZR, FANCA, ERCC4,
Protein Size: 2419

Molecular Weight: 266 kDa

Gene ID: 6708

NCBI Accession: NM_003126, NP_003117

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization