SIRPA antibody (C-Term)

Catalog No. ABIN2792091

This Rabbit Polyclonal antibody specifically detects SIRPA in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Pig and Horse.

Quick Overview for SIRPA antibody (C-Term) (ABIN2792091)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Rat, Mouse, Cow, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIRPA Antibody

Binding Specificity: C-Term

Sequence: QTSPQPASED TLTYADLDMV HLNRTPKQPA PKPEPSFSEY ASVQVPRK

Predicted Reactivity: Cow: 92%, Horse: 84%, Human: 100%, Mouse: 100%, Pig: 90%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against SIRPA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SIRPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 504 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: SIRPA

Background: SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Alias Symbols: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Protein Interaction Partner: CCDC57, KRT40, TRIM2, TRIM27, KRT31, KRT15, UBC, GRB2, Htt, NOL3, IL1RAP, CD47, PTPN6, PTPN11, JAK2,
Protein Size: 504

Molecular Weight: 52 kDa

Gene ID: 140885

NCBI Accession: NM_080792, NP_542970

UniProt: B2R6C3