SMAD3 antibody (N-Term)

Catalog No. ABIN2792537

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for SMAD3 antibody (N-Term) (ABIN2792537)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD3 Antibody

Binding Specificity: N-Term

Sequence: FTPPIVKRLL GWKKGEQNGQ EEKWCEKAVK SLVKKLKKTG QLDELEKAIT

Cross-Reactivity: Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMAD3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD3

Application Details

Application Notes: WB Suggested Anti-SMAD3 Antibody Titration: 2.0 µg/mL
Positive Control: Human Liver.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 425 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases.
Alias Symbols: LDS1C, MADH3, JV15-2, HSPC193, HsT17436
Protein Interaction Partner: TEKT4, CCDC33, CPSF7, MEOX2, HDAC1, PHC2, WWP1, AES, BLZF1, UBC, TP53, SMAD4, BACH1, MAFK, FOXO3, NEDD8, TRIM33, WWP2, SMURF2, FOXM1, TRIM62, TP63, OTUB1, NEDD4L, BAG3, TGFB1I1, CSNK1D, RUNX1, HCVgp1, TSC2, FHL3, FHL2, FHL1, Melk, IRF7, RNF111, LCK, KLF5,
Protein Size: 425

Molecular Weight: 48 kDa

Gene ID: 4088

NCBI Accession: NM_005902, NP_005893

UniProt: Q92940

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy