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SMAD3 antibody (N-Term)

Name: SMAD3 antibody (N-Term)
SKU: ABIN2792537
Price: 429.69 USD
Availability: InStock

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Dog and Guinea Pig.

WB Suggested Anti-SMAD3 Antibody Titration: 2. (SMAD3 antibody (N-Term))

Human Pancrease (SMAD3 antibody (N-Term))

IHC

Host: Mouse Target Name: SMAD3 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (SMAD3 antibody (N-Term))

Rabbit Anti-SMAD3 Antibody Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (SMAD3 antibody (N-Term))

IHC

4 images

Catalog No. ABIN2792537

Quick Overview for SMAD3 antibody (N-Term) (ABIN2792537)

Target

See all SMAD3 Antibodies

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity

Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Guinea Pig

Host

Rabbit

Clonality

Polyclonal

Conjugate

This SMAD3 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product SMAD3 Primary Antibody

Binding Specificity
40

34

27

27

25

12

11

8

6

6

6

5

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term

Sequence

FTPPIVKRLL GWKKGEQNGQ EEKWCEKAVK SLVKKLKKTG QLDELEKAIT

Cross-Reactivity

Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity

Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics

This is a rabbit polyclonal antibody against SMAD3. It was validated on Western Blot and immunohistochemistry.

Purification

Protein A purified

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD3
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215) antibody
SMAD3 Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB24758 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
KO Validated SMAD3 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

WB Suggested Anti-SMAD3 Antibody Titration: 2.0 µg/mL
Positive Control: Human Liver.
Optimal working dilutions should be determined experimentally by the investigator.

Comment

Antigen size: 425 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

-20 °C

Storage Comment

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name

SMAD3

Background

SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases.
Alias Symbols: LDS1C, MADH3, JV15-2, HSPC193, HsT17436
Protein Interaction Partner: TEKT4, CCDC33, CPSF7, MEOX2, HDAC1, PHC2, WWP1, AES, BLZF1, UBC, TP53, SMAD4, BACH1, MAFK, FOXO3, NEDD8, TRIM33, WWP2, SMURF2, FOXM1, TRIM62, TP63, OTUB1, NEDD4L, BAG3, TGFB1I1, CSNK1D, RUNX1, HCVgp1, TSC2, FHL3, FHL2, FHL1, Melk, IRF7, RNF111, LCK, KLF5,
Protein Size: 425

Molecular Weight

48 kDa

Gene ID

4088

NCBI Accession

NM_005902, NP_005893

UniProt

Q92940

Pathways

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy