HSF1 antibody (C-Term)

Catalog No. ABIN2792576

The Rabbit Polyclonal anti-HSF1 antibody has been validated for WB. It is suitable to detect HSF1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Dog and Pig.

Quick Overview for HSF1 antibody (C-Term) (ABIN2792576)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSF1 Antibody

Binding Specificity: C-Term

Sequence: SVDTGSNDLP VLFELGEGSY FSEGDGFAED PTISLLTGSE PPKAKDPTVS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 85%, Pig: 100%, Rabbit: 92%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against HSF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HSF1

Application Details

Application Notes: WB Suggested Anti-HSF1 Antibody Titration: 0.0625 µg/mL
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 529 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: Heat Shock Factor 1 / HSF1

Background: HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.
Alias Symbols: HSTF1
Protein Interaction Partner: HSP90AA1, SUMO2, SUMO3, NME2, KPNA4, KPNA3, HSPB1, HSF4, HSF2, HSPBP1, HSPA4L, BAG3, UBC, CBS, CALU, AARS, AAMP, TPRKB, PLIN3, ARPC1B, KIAA0101, MTMR2, ZPR1, UBE3A, SURF2, SRP9, HSPA8, DR1, DHPS, DTD1, BTF3L4, LRSAM1, C14orf142, GOPC, ANKMY2, CTPS2, RPRD1
Protein Size: 529

Molecular Weight: 57 kDa

Gene ID: 3297

NCBI Accession: NM_005526, NP_005517

UniProt: Q00613