SOX9 antibody (N-Term)

Catalog No. ABIN2792587

The Rabbit Polyclonal anti-SOX9 antibody (ABIN2792587) specifically detects SOX9 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio) and Goat samples.

Quick Overview for SOX9 antibody (N-Term) (ABIN2792587)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SOX9 Antibody

Binding Specificity: N-Term

Sequence: PCPSGSGSDT ENTRPQENTF PKGEPDLKKE SEEDKFPVCI REAVSQVLKG

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SOX9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SOX9

Application Details

Application Notes: WB Suggested Anti-SOX9 Antibody Titration: 2.5 µg/mL
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Alias Symbols: CMD1, SRA1, CMPD1
Protein Interaction Partner: ZBTB7A, SCX, HERC1, UBC, SOX9, ATP6V1H, ctnnb1-a, RUNX2, SMAD3, SMAD2, EP300, CREB3L4, SPEN, KPNB1, TRAF2, CREBBP, MED12, NR5A1, MAF, HSPA1A, CREB1,
Protein Size: 509

Molecular Weight: 56 kDa

Gene ID: 6662

NCBI Accession: NM_000346, NP_000337

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development