TAF1A antibody (C-Term)

Catalog No. ABIN2792590

There is 1 publication for this product available. The Rabbit Polyclonal anti-TAF1A antibody is suitable to detect TAF1A in samples from Human, Rat, Mouse, Horse, Rabbit, Pig, Dog and Cow. It has been validated for WB and IHC.

Quick Overview for TAF1A antibody (C-Term) (ABIN2792590)

Target: TAF1A (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A))

Reactivity: Human, Rat, Mouse, Horse, Rabbit, Pig, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TAF1A Antibody

Binding Specificity: C-Term

Sequence: CEKAFVAGLL LGKGCRYFRY ILKQDHQILG KKIKRMKRSV KKYSIVNPRL

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 78%, Dog: 85%, Horse: 85%, Human: 100%, Mouse: 85%, Pig: 85%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against TAF1A. It was validated on Western Blot using a cell lysate as a positive control.

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TAF1A

Application Details

Application Notes: WB Suggested Anti-TAF1A Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 336 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TAF1A antibody (ABIN2792590)

Di Pietro, Rapisarda, Amico, Bonaiuto, Viola, Scalia, Motta, Amato, Engel, Messina, Sichel, Grzeschik, Purrello: "Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1." in: Cytogenetics and cell genetics, Vol. 89, Issue 1-2, pp. 133-6, (2000) (PubMed).

Target Details for TAF1A

Target: TAF1A (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A))

Alternative Name: TAF1A

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. TAF1A encodes the smallest SL1-specific TAF.
Alias Symbols: SL1, RAFI48, TAFI48, MGC:17061
Protein Interaction Partner: C11orf57, JMJD6, TBP, Taf1c, Taf1b, BKRF1, UBC, TAF12, TAF1D, HIST1H4A, HIST1H3A, HIST2H2BE, HIST2H2AC, ESR1, SET, TAF1A, RUNX2, TP53, CD3EAP, HIST4H4, HIST3H3, ANP32A, UBTF, RRN3, MYC,
Protein Size: 336

Molecular Weight: 40 kDa

Gene ID: 9015

NCBI Accession: NM_139352, NP_647603

UniProt: Q9NWA1