GTF2F1 antibody (N-Term)

Catalog No. ABIN2792594

This Rabbit Polyclonal antibody specifically detects GTF2F1 in WB. It exhibits reactivity toward Human, Mouse, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for GTF2F1 antibody (N-Term) (ABIN2792594)

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Reactivity: Human, Mouse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GTF2F1 Antibody

Binding Specificity: N-Term

Sequence: MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GTF2F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2F1

Application Details

Application Notes: WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 µg/mL
Positive Control: K562 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 517 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2F1

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Alternative Name: GTF2F1 / RAP74

Background: GTF2F1 is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Alias Symbols: BTF4, RAP74, TF2F1, TFIIF
Protein Interaction Partner: ABL1, UBC, tat, PUS1, DUS3L, VPS29, SNF8, PSAP, BCAT1, TCEA1, GTF2B, POLR2C, GTF2F2, CDC73, MMS19, SF3A3, SRSF9, COIL, SNRPA, POLR2A, EEF1B2, CIRBP, CDK9, POLR2D, GTF2H1, GTF2E1, ERCC2, CDK8, CCNC, MAGEC1, TAF1, HNRNPU, FCP1, CSNK2A1, CCNT1, Gtf3c6, POLR2
Protein Size: 517

Molecular Weight: 58 kDa

Gene ID: 2962

NCBI Accession: NM_002096, NP_002087

UniProt: P35269