GATA1 antibody (N-Term)

Catalog No. ABIN2792607

The Rabbit Polyclonal anti-GATA1 antibody has been validated for WB. It is suitable to detect GATA1 in samples from Human, Mouse, Rat, Dog, Sheep, Cow, Rabbit and Guinea Pig. There is 1 publication available.

Quick Overview for GATA1 antibody (N-Term) (ABIN2792607)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat, Dog, Sheep, Cow, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GATA1 Antibody

Binding Specificity: N-Term

Sequence: SGPEGLDAAA SSTAPSTATA AAAALAYYRD AEAYRHSPVF QVYPLLNCME

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against GATA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GATA1

Application Details

Application Notes: WB Suggested Anti-GATA1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 413 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GATA1 antibody (ABIN2792607)

de Vooght, van Wijk, van Solinge: "GATA-1 binding sites in exon 1 direct erythroid-specific transcription of PPOX." in: Gene, Vol. 409, Issue 1-2, pp. 83-91, (2008) (PubMed).

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1

Background: GATA1 is a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ERYF1, GF1, NFE1, GF-1, XLTT, GATA-1
Protein Interaction Partner: KRTAP10-5, CCDC24, HEXIM2, FBF1, KRTAP9-2, RADIL, FRS3, PRKAB2, HSPB1, HOXA1, AKT1, SPIB, SPI1, PRKAA1, HSPA4, FLI1, CASP3, SRA1, SUMO1, CEBPE, MAPK6, UBC, SKI, NME1, RAI1, ZBTB22, TRIM25, TAF7, TAX1BP3, ZZZ3, TRIM29, IKZF1, Rb1, ZFPM1, ZNF521, ZBTB16, HD
Protein Size: 413

Molecular Weight: 43 kDa

Gene ID: 2623

NCBI Accession: NM_002049, NP_002040

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin