Retinoic Acid Receptor alpha antibody (Middle Region)

Catalog No. ABIN504661

This Rabbit Polyclonal antibody specifically detects Retinoic Acid Receptor alpha in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Rabbit.

Quick Overview for Retinoic Acid Receptor alpha antibody (Middle Region) (ABIN504661)

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoic Acid Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Retinoic Acid Receptor alpha Antibody

Binding Specificity: Middle Region

Purpose: RARA Antibody - middle region

Sequence: EMLENSEGLD TLSGQPGGGG RDGGGLAPPP GSCSPSLSPS SNRSSPATHS

Cross-Reactivity: Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against RARA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RARA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: RARA

Background:

Background Information: Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor, which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1, SMRT, and histone deacetylase. When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases, and the basic transcription machinery.

Gene Name: Retinoic acid receptor, alpha

Alternative Symbols: RAR, NR1B1

Protein Name: Retinoic acid receptor alpha

Molecular Weight: 51kDa

Gene ID: 5914

NCBI Accession: NP_000955

UniProt: P10276

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins