RUNX1 antibody (Middle Region)

Catalog No. ABIN2792628

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for RUNX1 antibody (Middle Region) (ABIN2792628)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX1 Antibody

Binding Specificity: Middle Region

Sequence: CTNASTGSAL LNPSLPNQSD VVEAEGSHSN SPTNMAPSAR LEEAVWRPY

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against RUNX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX1

Application Details

Application Notes: WB Suggested Anti-RUNX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 480 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. RUNX1 is the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with severaltypes of leukemia. Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
Protein Interaction Partner: YAP1, UBC, HDAC11, SOX2, SMAD3, CTBP1, RUNX1, FOXP3, NCOR2, MYC, NOTCH1, UXT, CTBP2, KAT6A, PML, CREBBP, CBFB, PRMT1, HLA-DMA, HDAC2, HDAC1, CBFA2T3, RUNX1T1, CBFA2T2, ELAVL1, DNMT1, SPEN, RBM14, KMT2A, COPRS, HDAC3, TAL1, CDK1, SMARCC1, SMARCB1, SMARCA4,
Protein Size: 480

Molecular Weight: 52 kDa

Gene ID: 861

NCBI Accession: NM_001754, NP_001745

UniProt: B2RMS4