OCT4 antibody (N-Term)

Catalog No. ABIN2792671

This Rabbit Polyclonal antibody specifically detects OCT4 in WB. It exhibits reactivity toward Human, Pig, Dog, Rabbit and Goat. It has been mentioned in 3+ publications

Quick Overview for OCT4 antibody (N-Term) (ABIN2792671)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Pig, Dog, Rabbit, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OCT4 Antibody

Binding Specificity: N-Term

Purpose: POU5F1 Antibody - N-terminal region

Sequence: PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Pig (Porcine), Rabbit

Predicted Reactivity: Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%

Characteristics: This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-OCT4 antibody (ABIN2792671)

Li, Moretto-Zita, Soncin, Wakeland, Wolfe, Leon-Garcia, Pandian, Pizzo, Cui, Nazor, Loring, Crum, Laurent, Parast: "BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).

Kaeffer, Legrand, Moyon, Frondas-Chauty, Billard, Guzman-Quevedo, Darmaun, Rozé: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." in: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).

Suo, Han, Wang, Zhang, Zhao, Zhao, Dai: "Oct4 pseudogenes are transcribed in cancers." in: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background:

Background Information: POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm

Gene Name: POU class 5 homeobox 1

Alternative Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4

Protein Name: POU domain, class 5, transcription factor 1

Molecular Weight: 39kDa

Gene ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance